Chemical Composition and Effect of Aqueous Extract of Alpinia oxyphylla Miq. on Inhibition of Salmonella typhi

Xiaoyun Zhang; Dong Liu; Qichang Yang; Shiyu Jin; Xiaojie Zhou

doi:10.62177/apjcmr.v1i5.859

Authors

Xiaoyun Zhang Hainan Vocational University of Science and Technology
Dong Liu Tongchuan Hengyuan Guangming Pharmaceutical Compnay https://orcid.org/0000-0002-2827-4754
Qichang Yang Beijing Rehabilitation Hospital Affiliated to Capital Medical University
Shiyu Jin Huai'an University
Xiaojie Zhou Hainan Vocational University of Science and Technology

DOI:

https://doi.org/10.62177/apjcmr.v1i5.859

Keywords:

Alpinia oxyphylla Miq., Salmonella typhi, Extraction, Antibacterial Activity

Abstract

Objective: To investigate the separation and extraction process of the primary chemical constituents of Alpinia oxyphylla Miq. against Salmonella typhi, and its antibacterial efficacy, providing theoretical basis for novel gastrointestinal epidemic drugs and interventions against pathogen transmission. Methods: Alpinia oxyphylla Miq.: purified water-to-material ratio 1:5, refluxed at 100°C for 6 hours, repeated three times. Alpinia oxyphylla Miq. extract was concentrated by freeze-drying at a 30:1 solid-to-liquid ratio to obtain the final extract. The efficacy of the extraction method and antibacterial activity were assessed using the disk diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined to establish the minimum therapeutic thresholds. Gas chromatography-mass spectrometry (GCMS) was employed to identify the primary bioactive constituents and their relative concentrations in the Alpinia oxyphylla Miq. aqueous extract. Results: The water extract of Alpinia oxyphylla Miq. Miq. demonstrated potent antibacterial activity against Salmonella typhi (P < 0.001), with an inhibition zone diameter of 8.43 ± 0.11 mm, MIC of 0.25 g/ml, and MBC of 0.5 g/ml. The extraction process was established as follows: Alpinia oxyphylla Miq. : purified water ratio 1:5, reflux heating at 100°C for 6 hours, repeated three times, followed by freeze-drying at 30:1 concentration. This yielded an extract rich in , primarily comprising octadecanoic acid (44.27%), palmitic acid (26.18%), caproic acid (8.15%), 10-hydroxydecanoic acid (7.83%) as the main components, supplemented by 3-hydroxy-dodecanoic acid (1.78%), cis-13-eicosenoic acid (1.58%), valeric acid (1.21%), heptanoic acid (1.13%), nonanoic acid (1.06%), oleic acid (0.87%), and butyric acid (0.47%). Conclusion: This extraction process for novel antimicrobial compounds from Alpinia oxyphylla Miq. demonstrates high stability, operational feasibility, and precise composition and efficacy. It provides a practical theoretical foundation for developing novel natural medicines against infectious diseases caused by Salmonella typhi, while offering further insights for antimicrobial drug development.

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Author Biography

Dong Liu, Tongchuan Hengyuan Guangming Pharmaceutical Compnay

Director/Principal, Dalian Phytobenthic Biomedical Innovation Center

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